Control | 100 μM Adenosine | Wash | P | |
---|---|---|---|---|
Layer 2 pyramidal neurons (n = 8) | ||||
RMP (mV) | -76.5 ± 1.5 | -76.4 ± 1.3 | -77.0 ± 1.3 | 0.73 |
Input resistance (MΩ) | 160 ± 14 | 173 ± 17 | 164 ± 16 (n = 7) | <0.01/0.58 |
Time constant (ms) | 20.5 ± 1.4 | 23.4 ± 1.2 | 24.9 ± 1.7 (n = 7) | 0.07/0.37 |
Rheobase (pA) | 186 ± 12 | 188 ± 13 | 191 ± 14 (n = 7) | 0.86 |
Layer 3 pyramidal neurons | ||||
Responding (n = 8) | ||||
RMP (mV) | -67.5 ± 0.8 | -70.9 ± 0.8 | -67.6 ± 1.0 | <0.01/<0.01 |
Input resistance (MΩ) | 138 ± 11 | 116 ± 11 | 140 ± 11 | 0.02/<0.01 |
Time constant (ms) | 29.3 ± 2.9 | 24.6 ± 2.0 | 29.3 ± 3.4 | 0.056/0.18 |
Rheobase (pA) | 124 ± 11 | 175 ± 18 | 119 ± 10 | 0.01/<0.01 |
Nonresponding (n = 4) | ||||
RMP (mV) | -78.9 ± 1.3 | -79.2 ± 1.4 | -79.7 ± 1.6 | 0.23 |
Input resistance (MΩ) | 142 ± 16 | 150 ± 18 | 150 ± 14 | 0.04/0.96 |
Time constant (ms) | 18.5 ± 0.7 | 17.2 ± 1.9 | 19.7 ± 3.3 | 0.41 |
Rheobase (pA) | 233 ± 15 | 228 ± 26 | 230 ± 11 | 0.70 |
Layer 5 pyramidal neurons (n = 20) | ||||
RMP (mV) | -64.6 ± 0.5 (n = 30) | -68.4 ± 0.6 (n = 30) | -64.3 ± 0.6 (n = 28) | <0.01/<0.01 |
Input resistance (MΩ) | 208 ± 25 | 178 ± 24 | 224 ± 31 | <0.01/<0.01 |
Time constant (ms) | 35.0 ± 1.9 (n = 18) | 28.4 ± 1.6 (n = 18) | 38.6 ± 2.7 (n = 18) | <0.01/<0.01 |
Rheobase (pA) | 96 ± 9 | 145 ± 14 | 90 ± 10 | <0.01/<0.01 |
Layer 6 pyramidal neurons (n = 6) | ||||
RMP (mV) | -70.1 ± 1.9 (n = 10) | -74.4 ± 1.8 (n = 10) | -67.3 ± 1.5 (n = 8) | <0.01/<0.01 |
Input resistance (MΩ) | 301 ± 46 | 272 ± 40 | 400 ± 77 (n = 5) | 0.31/0.03 |
Time constant (ms) | 26.1 ± 4.0 | 20.7 ± 2.2 | 40.2 ± 5.0 (n = 5) | 0.21/0.02 |
Rheobase (pA) | 93 ± 22 | 120 ± 19 | 54 ± 14 (n = 5) | 0.10/<0.01 |
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Note: Average ± SEM; RMP, resting membrane potential. P lists the results from paired t-tests between control and adenosine. When P < 0.10 the result from the paired t-test between adenosine and wash-out is also given. The number of cells for the calculation of passive properties is generally lower than for the RMP to allow an uninterrupted continuous recording of the RMP in a subset of experiments.